Gene name |
SPAC1071.02 |
Gene ID |
30/C05 |
Gene synonyms/obsolete |
|
Gene product |
involved in DNA
repair; nucleotide-excision repair; interacts with
transcription factor TFIIH |
Entry clone |
Cloned |
ORF length (unspliced) |
3257 |
ORF length (spliced) |
3057 |
Entry clone length |
3257 |
No. of intron |
4 |
Sequence status |
Finished |
Sequence results |
573A:G / 745G:A /
1519T:C / 3059A:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1071.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCTCGAATTTAGTGGC |
Rev primer name |
SPAC1071.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATATATACCACTTATGACGA |
Amino acid length |
1018 |
Molecular weight |
114.6 |
Isoelectric point (calc.) |
5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEYVYKCLVL/LHFIEKLNI/LAFKLLDTLNL/LPLLLQCLSL/LDTLIPSLLAI |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |