| Gene name |
SPAC14C4.02c |
| Gene ID |
30/C03 |
| Gene synonyms/obsolete |
spr18 |
| Gene product |
SMC family protein;
possibly dimerizes with rad18 |
| Entry clone |
Cloned in 2006
trial |
| ORF length (unspliced) |
3247 |
| ORF length (spliced) |
3198 |
| Entry clone length |
3247 |
| No. of intron |
1 |
| Sequence status |
Partially
sequenced |
| Sequence results |
100% match in both
ends |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC14C4.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATCTTAACACGCGAGAG |
| Rev primer name |
SPAC14C4.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGACGAAGAAATGAGTGCG |
| Amino acid length |
1065 |
| Molecular weight |
122.9 |
| Isoelectric point (calc.) |
5.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVALCHMLKI/LLSLQGLAI |
| Localization (YFP) |
SPB; cytosol=nucleus;
nuclear dots; periphery at site of septum formation; spindle
microtubules |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |