| Gene name |
SPBC1709.02c |
| Gene ID |
30/B09 |
| Gene synonyms/obsolete |
SPBC1734.18c |
| Gene product |
valine-tRNA ligase;
involved in valyl-tRNA aminoacylation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3173 |
| ORF length (spliced) |
2943 |
| Entry clone length |
3173 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1709.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAGACAAAGGCTGTGA |
| Rev primer name |
SPBC1709.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTTGTTTCGTTCCAAAAAG |
| Amino acid length |
980 |
| Molecular weight |
111.3 |
| Isoelectric point (calc.) |
6.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYTVLDNALRL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |