| Gene name |
SPBC28E12.03 |
| Gene ID |
30/B03 |
| Gene synonyms/obsolete |
rga4 |
| Gene product |
GTPase activator;
RhoGAP domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3144 |
| ORF length (spliced) |
2802 |
| Entry clone length |
3144 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC28E12.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATTCGGGTACGACACT |
| Rev primer name |
SPBC28E12.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGGCAAAGACTTCATGTACA |
| Amino acid length |
933 |
| Molecular weight |
104.4 |
| Isoelectric point (calc.) |
8.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQAEVGTLLL |
| Localization (YFP) |
periphery |
| Comments for localization |
except cell tip and
site of septum formation |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |