| Gene name |
SPCC1739.11c |
| Gene ID |
30/B01 |
| Gene synonyms/obsolete |
cdc11 |
| Gene product |
involved in septation;
SIN component; leucine-rich repeat protein; localizes
components of the SIN to the SPB; phosphoprotein;
essential |
| Entry clone |
Cloned; Mixture |
| ORF length (unspliced) |
3138 |
| ORF length (spliced) |
|
| Entry clone length |
3138 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC1739.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAACAGTTATGGCTTGA |
| Rev primer name |
SPCC1739.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTTGGATGATTTGGTGAAT |
| Amino acid length |
1045 |
| Molecular weight |
118.6 |
| Isoelectric point (calc.) |
6.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSSFHDLSL/LCPSIEELTL/LTSFSNLLNL/LHRLEELLL/LQNLMVLQL/LQMIHRLYL/LKQLNVLDL |
| Localization (YFP) |
SPB; nuclear dots;
periphery at site of septum formation; cytoplasmic dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |