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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC664.10
Gene ID 29/A08
Gene synonyms/obsolete
Gene product kinesin-like protein
Entry clone Cloned
ORF length (unspliced) 2499
ORF length (spliced) 2454
Entry clone length 2499
No. of intron 1
Sequence status Finished
Sequence results 18T:A / 752A:G / 969A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC664.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAGAAGAAGGACATAA
Rev primer name SPAC664.10.Rv
Rev primer SEQ AGAAAGCTGGGTATTTTGTGACTTTGCGTGCT
Amino acid length 817
Molecular weight 91
Isoelectric point (calc.) 7.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) microtubules
Comments for localization
Effect of LMB on protein localization changed to: intranuclear microtubule bundle (nucleus)
Microscope used for observation Confocal

Image information
YFP 2 images) See all images
LMB 2 images) See all images

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