| Gene name |
SPAC2G11.09 |
| Gene ID |
28/H07 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved eukaryotic
protein; Sc RSN1 is involved in tunicamycin sensitivity;
DUF221 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2475 |
| ORF length (spliced) |
2391 |
| Entry clone length |
2475 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
1051A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC2G11.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTATTTTCGACAGTGAT |
| Rev primer name |
SPAC2G11.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCGGTGTTCAAATCAATT |
| Amino acid length |
796 |
| Molecular weight |
90.9 |
| Isoelectric point (calc.) |
8.5 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
9 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPTLVYSLFI/LCYSIISPLIL |
| Localization (YFP) |
vacuole membrane |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |