Gene name |
SPAC23C4.17 |
Gene ID |
28/B11 |
Gene synonyms/obsolete |
|
Gene product |
methyltransferase;
NOL1/NOP2/sun family; involved in methylation of cytidine to
5-methyl-cytidine (m5C) at several positions in different
tRNAs |
Entry clone |
Cloned |
ORF length (unspliced) |
2280 |
ORF length (spliced) |
2025 |
Entry clone length |
2280 |
No. of intron |
4 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC23C4.17.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGGAAAAGAAATAAAAA |
Rev primer name |
SPAC23C4.17.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTGCTTCTCTTTTCTAGCA |
Amino acid length |
674 |
Molecular weight |
76.6 |
Isoelectric point (calc.) |
8.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKSVEGLVI/LRILIRGLQL/LVDVSKKLPL |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica;
DeltaVision |