| Gene name |
SPAC13G6.03 |
| Gene ID |
28/B09 |
| Gene synonyms/obsolete |
|
| Gene product |
involved in
glycosylphospatidylinositol modification; phosphoethanolamine
N-methyltransferase activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2277 |
| ORF length (spliced) |
|
| Entry clone length |
2277 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1381G:A / 1535C:T /
2040A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC13G6.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGGTTTGCATTTTTTGG |
| Rev primer name |
SPAC13G6.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATCGTAAACGGACCATGATG |
| Amino acid length |
758 |
| Molecular weight |
85.6 |
| Isoelectric point (calc.) |
6.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
10 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQIFGSILFL/LLNIKLLEI/LSNLFQLSL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |