| Gene name |
SPAC6F12.17 |
| Gene ID |
28/A11 |
| Gene synonyms/obsolete |
|
| Gene product |
HAT repeat protein
(SMART); involved in polyadenylation; involved in 3' end
maturation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2245 |
| ORF length (spliced) |
2202 |
| Entry clone length |
2245 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC6F12.17.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATTCTGATGACAATGT |
| Rev primer name |
SPAC6F12.17.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTAAATCTGGCACGCTTC |
| Amino acid length |
733 |
| Molecular weight |
83.9 |
| Isoelectric point (calc.) |
6.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSVDLWTLYL/LDGFSYFLSI |
| Localization (YFP) |
nuclear dots;
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
| Microscope used for
observation |
DeltaVision |