| Gene name |
SPAC3F10.01 |
| Gene ID |
28/A08 |
| Gene synonyms/obsolete |
nda4; mcm5;
SPAC1B2.05 |
| Gene product |
MCM complex subunit;
AAA family ATPase; involved in DNA replication (initiation)
(required); ATP dependent; DNA helicase activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2244 |
| ORF length (spliced) |
2163 |
| Entry clone length |
2244 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1559A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC3F10.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTGCAGCAACAGGTTG |
| Rev primer name |
SPAC3F10.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATACTCCTGATCGATACACC |
| Amino acid length |
720 |
| Molecular weight |
80 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRGDINVLLL |
| Localization (YFP) |
cytosol; cytoplasmic
dots by over expression |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
| Microscope used for
observation |
Leica |