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Schizosaccharomyces pombe Postgenome Database

Gene name SPAPYUG7.03c
Gene ID 28/A03
Gene synonyms/obsolete mid2
Gene product pleckstrin homology domain; involved in cytokinesis; involved in septation; involved in cytokinesis, formation of actomyosin apparatus; involved in cell separation; GTP-binding protein (ISS)
Entry clone Cloned
ORF length (unspliced) 2115
ORF length (spliced)
Entry clone length 2115
No. of intron 0
Sequence status Finished
Sequence results 332T:C / 935T:C / 1472T:C / 2046T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAPYUG7.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTGCTTCTCAACAGGA
Rev primer name SPAPYUG7.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAACTCAGCGAAAGAAAT
Amino acid length 704
Molecular weight 78.7
Isoelectric point (calc.) 5.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LADIHRALRI/LMEIRNLTI/LPRKVGELEI
Localization (YFP) periphery at site of septum formation; cytosol; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization changed to: nucleus>cytosol; cytoplasmic dots
Microscope used for observation Leica

Image information
YFP 1 images) See all images
LMB 4 images) See all images

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