| Gene name |
SPBC23G7.08c |
| Gene ID |
27/G09 |
| Gene synonyms/obsolete |
rga7 |
| Gene product |
GTPase activator;
RhoGAP domain; actin cortical patch component |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2088 |
| ORF length (spliced) |
|
| Entry clone length |
2088 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
716T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC23G7.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTGTCGGCTCCCTCCTC |
| Rev primer name |
SPBC23G7.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCGTAGTCAAAAATTGTG |
| Amino acid length |
695 |
| Molecular weight |
76.6 |
| Isoelectric point (calc.) |
8.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
141 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol; periphery at
site of septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|