| Gene name |
SPCC1450.09c |
| Gene ID |
27/D10 |
| Gene synonyms/obsolete |
|
| Gene product |
lysophospholipase;
similar to Sp SPAC1A6.03C and SPAC1A6.04C and SPAC1348.10C and
SPAC1786.02 and SPAC977.09C |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2009 |
| ORF length (spliced) |
1902 |
| Entry clone length |
2009 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
1384T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1450.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAATTATCTTCCTTTGG |
| Rev primer name |
SPCC1450.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGAGCAATAAGTAAAGAGC |
| Amino acid length |
633 |
| Molecular weight |
68.2 |
| Isoelectric point (calc.) |
4.1 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSSFGLFLAL |
| Localization (YFP) |
Golgi with
accumulation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |