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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC320.05
Gene ID 27/D07
Gene synonyms/obsolete
Gene product sulfate transporter
Entry clone Cloned
ORF length (unspliced) 2004
ORF length (spliced)
Entry clone length 2004
No. of intron 0
Sequence status Finished
Sequence results 431A:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC320.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGTCTCCTTCAGAAAA
Rev primer name SPCC320.05.Rv
Rev primer SEQ AGAAAGCTGGGTACACTCCAAGTTCGATATCG
Amino acid length 667
Molecular weight 73.4
Isoelectric point (calc.) 8.7
Signal SEQ
No. of transmembrane domain 10
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSCITISLLI/LETGIIFGLSI
Localization (YFP) ER; vacuole membrane
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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