Gene name |
SPBC28E12.02 |
Gene ID |
27/D02 |
Gene synonyms/obsolete |
SPBC9B6.13 |
Gene product |
conserved hypothetical
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1992 |
ORF length (spliced) |
|
Entry clone length |
1992 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC28E12.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTCTCATGTTTTAGC |
Rev primer name |
SPBC28E12.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTAGCAAACAAATACTTC |
Amino acid length |
663 |
Molecular weight |
75.4 |
Isoelectric point (calc.) |
6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAYEMDELRL/LWAVRSLFI |
Localization (YFP) |
cytosol=nucleus;
SPB? |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |