Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC2E12.01
Gene ID 27/C07
Gene synonyms/obsolete SPAC22E12.19
Gene product Myb family; DNA binding protein; predicted coiled-coil region
Entry clone Cloned
ORF length (unspliced) 1986
ORF length (spliced)
Entry clone length 1986
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC2E12.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATTTGACTACCCCGGA
Rev primer name SPAC2E12.01.Rv
Rev primer SEQ AGAAAGCTGGGTATACAATTTTATCGTTTTTG
Amino acid length 661
Molecular weight 75
Isoelectric point (calc.) 5.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 114
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus; nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.