| Gene name |
SPAC3F10.03 |
| Gene ID |
27/A09 |
| Gene synonyms/obsolete |
|
| Gene product |
glycine tRNA-ligase;
functionally complements Sc GRS1 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1959 |
| ORF length (spliced) |
|
| Entry clone length |
1959 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
17A:G / 998T:C /
1716T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC3F10.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACAGAAGTTTCAAAGGC |
| Rev primer name |
SPAC3F10.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCTTGTGTAGACTTAAAC |
| Amino acid length |
652 |
| Molecular weight |
74 |
| Isoelectric point (calc.) |
5.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
138/137 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSAKLRNLGI |
| Localization (YFP) |
cytosol; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|