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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1773.01
Gene ID 26/G05
Gene synonyms/obsolete
Gene product WD repeat protein; striatin; involved in cell cycle progression; human ortholog is expressed mainly in S and G phase; possibly binds calmodulin
Entry clone Cloned
ORF length (unspliced) 1920
ORF length (spliced) 1839
Entry clone length 1920
No. of intron 2
Sequence status Finished
Sequence results 1140C:T / 1393C:T / 1530T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1773.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAATCCAACCTATCATT
Rev primer name SPBC1773.01.Rv
Rev primer SEQ AGAAAGCTGGGTATGGTAAATATTTGCGGTCG
Amino acid length 612
Molecular weight 69.4
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization bright large dots by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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