| Gene name |
SPBC660.15 |
| Gene ID |
26/F08 |
| Gene synonyms/obsolete |
|
| Gene product |
RNA-binding protein;
rrm RNA recognition motif; mRNA cleavage factor complex;
involved in polyadenylation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1911 |
| ORF length (spliced) |
1425 |
| Entry clone length |
1911 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
1314C:T / 1383C:T /
1386C:T / 1409C:T / 1439A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC660.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGCGGCTCTGATTTTGA |
| Rev primer name |
SPBC660.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATCGACGATATGGATGGAAG |
| Amino acid length |
474 |
| Molecular weight |
52.8 |
| Isoelectric point (calc.) |
4.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |