| Gene name |
SPAC27E2.01 |
| Gene ID |
26/A07 |
| Gene synonyms/obsolete |
|
| Gene product |
alpha-amylase; no
apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1629 |
| ORF length (spliced) |
1476 |
| Entry clone length |
1629 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
933T:A / 1156C:T /
1277G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC27E2.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTCTAGATAAACAAAT |
| Rev primer name |
SPAC27E2.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCCAGTTCATTCATGGAA |
| Amino acid length |
491 |
| Molecular weight |
56.6 |
| Isoelectric point (calc.) |
5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |