| Gene name |
SPAC458.02c |
| Gene ID |
26/A02 |
| Gene synonyms/obsolete |
|
| Gene product |
involved in mitosis;
involved in cytokinesis; involved in meiosis; involved in
spindle assembly |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1624 |
| ORF length (spliced) |
1407 |
| Entry clone length |
1624 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1178C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC458.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCATCTCATAAACCCGT |
| Rev primer name |
SPAC458.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCTGTAGCTTCCACCTCC |
| Amino acid length |
468 |
| Molecular weight |
53.7 |
| Isoelectric point (calc.) |
9.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |