| Gene name |
SPAC17A5.04c |
| Gene ID |
25/G12 |
| Gene synonyms/obsolete |
|
| Gene product |
zinc metallopeptidase;
disintegrin/ADAM protease; GPI anchored protein (pers. comm.
Birgit Eisenhaber); glycoprotein; non-essential; no apparent
Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1610 |
| ORF length (spliced) |
1539 |
| Entry clone length |
1610 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
1329T:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC17A5.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGGCTCGTTTTACTGTT |
| Rev primer name |
SPAC17A5.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACCAGAAAAACCATGCTATT |
| Amino acid length |
512 |
| Molecular weight |
56.4 |
| Isoelectric point (calc.) |
5.3 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFPALQTLWI |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |