| Gene name |
SPAC16C9.04c |
| Gene ID |
25/G07 |
| Gene synonyms/obsolete |
|
| Gene product |
basal transcriptional
regulator; zinc finger protein; zf-CCCH type; CCR4-Not
complex; rrm RNA recognition motif; RNA-binding protein |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
1608 |
| ORF length (spliced) |
1470 |
| Entry clone length |
1608 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC16C9.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTAAAAACGCAAGAGAT |
| Rev primer name |
SPAC16C9.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACTTTGGAAACCAGGAGGA |
| Amino acid length |
489 |
| Molecular weight |
54.4 |
| Isoelectric point (calc.) |
8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
88 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
| Microscope used for
observation |
DeltaVision |