Gene name |
SPCC18.08 |
Gene ID |
25/F05 |
Gene synonyms/obsolete |
|
Gene product |
lysyl-tRNA synthetase;
involved in lysyl-tRNA aminoacylation; lysine-tRNA ligase
activity |
Entry clone |
Cloned# |
ORF length (unspliced) |
1596 |
ORF length (spliced) |
|
Entry clone length |
1596 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC18.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTTCACGAGGTCTTTT |
Rev primer name |
SPCC18.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACATATCGAAGAGATCCA |
Amino acid length |
531 |
Molecular weight |
60.2 |
Isoelectric point (calc.) |
8.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIRFICLTI/LLDKLFDSLVL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |