| Gene name |
SPCC553.01c |
| Gene ID |
25/C07 |
| Gene synonyms/obsolete |
SPCC736.01c |
| Gene product |
conserved
hypothetical; hypothetical protein; possibly involved in
signal transduction, as the only detectable defect of the null
mutant of the S. cerevisiae homolog YGR042w is in
heterologous signal transduction |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2148 |
| ORF length (spliced) |
|
| Entry clone length |
2148 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1672T:C /
1807T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC553.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATACAAGTTCCAATGT |
| Rev primer name |
SPCC553.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATAAAGTCACCATCTTCG |
| Amino acid length |
715 |
| Molecular weight |
78 |
| Isoelectric point (calc.) |
9.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB; nuclear
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |