| Gene name |
SPBC83.15 |
| Gene ID |
25/B11 |
| Gene synonyms/obsolete |
|
| Gene product |
WD repeat protein;
involved in ribosome biogenesis and assembly |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1556 |
| ORF length (spliced) |
1164 |
| Entry clone length |
1556 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
841T:C / 945T:C /
1430T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC83.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAATTGCTTCTTGGTGA |
| Rev primer name |
SPBC83.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGTTTTATCCTCTTCGAA |
| Amino acid length |
387 |
| Molecular weight |
43.7 |
| Isoelectric point (calc.) |
6.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |