| Gene name |
SPAC22A12.16 |
| Gene ID |
24/H08 |
| Gene synonyms/obsolete |
|
| Gene product |
ATP-citrate synthase
(subunit 2) (similar to ATP-citrate synthase N-terminal
domain); involved in tricarboxylic acid cycle; involved in
citrate metabolism; involved in ATP catabolism; involved in
acetyl CoA biosynthesis; involved in fatty acid biosynthesis;
ATP citrate synthase activity; no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1538 |
| ORF length (spliced) |
1479 |
| Entry clone length |
1538 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC22A12.16.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGCCAAGTCAATTAG |
| Rev primer name |
SPAC22A12.16.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCCATCTGCCATTCAGCT |
| Amino acid length |
492 |
| Molecular weight |
53.9 |
| Isoelectric point (calc.) |
5.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |