| Gene name |
SPBP8B7.17c |
| Gene ID |
24/F08 |
| Gene synonyms/obsolete |
|
| Gene product |
TENA/THI domain;
similar to Sp SPBP8B7.18C and SPCC18B5.05C (paralogs); tandem
duplication in pB8B7 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1521 |
| ORF length (spliced) |
|
| Entry clone length |
1521 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
568T:C / 1235G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBP8B7.17.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTTCGACTTGTATCAC |
| Rev primer name |
SPBP8B7.17.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAACACAGATGATTCGTTA |
| Amino acid length |
506 |
| Molecular weight |
55.7 |
| Isoelectric point (calc.) |
6.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLVMPDYLEL/LIPNIAEALII/LSSAVASNLAI/LSKVQHLLGI |
| Localization (YFP) |
cytosol=nucleus;
cytoplasmic dots |
| Comments for localization |
one nuclear dot |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |