Gene name |
SPAC22H10.05c |
Gene ID |
24/F01 |
Gene synonyms/obsolete |
|
Gene product |
involved in cleavage
and polyadenylation; CF IA component |
Entry clone |
Cloned |
ORF length (unspliced) |
1516 |
ORF length (spliced) |
1371 |
Entry clone length |
1516 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
94T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC22H10.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAGAAATTTTCATTCC |
Rev primer name |
SPAC22H10.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCCTGCCATTTGCAAGTT |
Amino acid length |
456 |
Molecular weight |
50.4 |
Isoelectric point (calc.) |
4.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LHFALEGLRL |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |