| Gene name |
SPBC12C2.06 |
| Gene ID |
24/E05 |
| Gene synonyms/obsolete |
|
| Gene product |
DEAD/DEAH box
helicase; ATP-dependent; RNA helicase; involved in mRNA expor
|
| Entry clone |
Cloned |
| ORF length (unspliced) |
1512 |
| ORF length (spliced) |
|
| Entry clone length |
1512 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC12C2.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAACTACACTGGGACA |
| Rev primer name |
SPBC12C2.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACATTTTAAGTGCATTCTTG |
| Amino acid length |
503 |
| Molecular weight |
56.3 |
| Isoelectric point (calc.) |
5.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVELYGLLTI |
| Localization (YFP) |
cytosol; nuclear
envelope |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol; nuclear envelope |
| Microscope used for
observation |
Leica |