| Gene name |
SPAC23C4.12 |
| Gene ID |
23/D12 |
| Gene synonyms/obsolete |
hhp2 |
| Gene product |
serine/threonine
protein kinase; involved in DNA repair (regulation); casein
kinase (implicated); similar to Sp hhp1 (paralog); function
partially overlapping with Hhp1p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1455 |
| ORF length (spliced) |
1203 |
| Entry clone length |
1455 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
536T:C / 1188A:G /
1288C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC23C4.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACGGTTGTTGACATTAA |
| Rev primer name |
SPAC23C4.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGGAGCTGGTTCTTCATCT |
| Amino acid length |
400 |
| Molecular weight |
45.8 |
| Isoelectric point (calc.) |
9.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRKLFRDLLI |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |