Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP8B7.15c
Gene ID 23/D03
Gene synonyms/obsolete
Gene product zinc finger protein; zf-CCHC type (zinc knuckle)
Entry clone Cloned
ORF length (unspliced) 1449
ORF length (spliced)
Entry clone length 1449
No. of intron 0
Sequence status Finished
Sequence results 501A:G / 1015A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP8B7.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGGTGTAATTTATTA
Rev primer name SPBP8B7.15.Rv
Rev primer SEQ AGAAAGCTGGGTAATTTGTTTTAGTAGGGGGA
Amino acid length 482
Molecular weight 53.1
Isoelectric point (calc.) 9.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LGNGLDFDLLL
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization changed to: intranuclear microtubule bundle (nucleus)
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images
LMB 4 images) See all images

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