Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC2H10.01
Gene ID 23/C04
Gene synonyms/obsolete
Gene product involved in transcriptional regulation; zinc finger protein; zf-fungal Zn(2)-Cys(6) binuclear cluster domain
Entry clone Cloned
ORF length (unspliced) 1443
ORF length (spliced)
Entry clone length 1443
No. of intron 0
Sequence status Finished
Sequence results 762A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC2H10.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTCAAAAAAATACCGG
Rev primer name SPAC2H10.01.Rv
Rev primer SEQ AGAAAGCTGGGTAATATGCAGTTTGAATTGGA
Amino acid length 480
Molecular weight 51.1
Isoelectric point (calc.) 5.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 8
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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