| Gene name |
SPAC31A2.01 |
| Gene ID |
23/C02 |
| Gene synonyms/obsolete |
csn71; csn7b; csn7;
csn7-2; SPAC1751.03 |
| Gene product |
COP9/signalosome
complex (subunit 7b); PCI domain; no apparent Sc
ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1443 |
| ORF length (spliced) |
1209 |
| Entry clone length |
1443 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC31A2.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAGGCTCTGATTTTAT |
| Rev primer name |
SPAC31A2.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCAGAGGCTGTGACCGCG |
| Amino acid length |
402 |
| Molecular weight |
45 |
| Isoelectric point (calc.) |
4.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNLFLTRLPL/LTPAILSVLSI |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |