Gene name |
SPBC16D10.10 |
Gene ID |
23/B06 |
Gene synonyms/obsolete |
|
Gene product |
tRNA specific
adenosine deaminase activity; involved in tRNA
modification |
Entry clone |
Cloned in 2006
trial |
ORF length (unspliced) |
1424 |
ORF length (spliced) |
1104 |
Entry clone length |
1424 |
No. of intron |
5 |
Sequence status |
Partially
sequenced |
Sequence results |
100% match in both
ends |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC16D10.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAGGAGATAGTGTGAA |
Rev primer name |
SPBC16D10.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTGAAACGAGATAAATCC |
Amino acid length |
367 |
Molecular weight |
41.3 |
Isoelectric point (calc.) |
7.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKREVKSLDL |
Localization (YFP) |
no expression clone
|
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
|