| Gene name |
SPBC30D10.03c |
| Gene ID |
22/H10 |
| Gene synonyms/obsolete |
|
| Gene product |
5'-nucleotidase
activity (IMP specific); involved in nucleotide
metabolism |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1317 |
| ORF length (spliced) |
1218 |
| Entry clone length |
1317 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
502T:C / 936T:C /
1273A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC30D10.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTTCTAGATATCGTGT |
| Rev primer name |
SPBC30D10.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTTTTTAAAAAAGAGAAA |
| Amino acid length |
405 |
| Molecular weight |
45.8 |
| Isoelectric point (calc.) |
5.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRSCIQGLNI |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |