| Gene name |
SPBC1709.12 |
| Gene ID |
22/G09 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
sequence orphan |
| Entry clone |
Cloned in 2004
trial |
| ORF length (unspliced) |
1310 |
| ORF length (spliced) |
1104 |
| Entry clone length |
1310 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC1709.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATTCTCTACGTTCAGT |
| Rev primer name |
SPBC1709.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACCTGCTCTTTCGAAACCGT |
| Amino acid length |
367 |
| Molecular weight |
42.5 |
| Isoelectric point (calc.) |
6.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
88 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LHDSILEQLLL/LINELLEGLPL |
| Localization (YFP) |
periphery at cell tip
and site of septum formation; cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus |
| Microscope used for
observation |
DeltaVision |