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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC869.02c
Gene ID 22/C04
Gene synonyms/obsolete
Gene product flavohemoprotein; involved in stress response
Entry clone Cloned
ORF length (unspliced) 1284
ORF length (spliced)
Entry clone length 1284
No. of intron 0
Sequence status Finished
Sequence results 869A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC869.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCCAGTGTTGAGGTTAA
Rev primer name SPAC869.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAGGAATATACGGTCCAAAA
Amino acid length 427
Molecular weight 48.4
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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