| Gene name |
SPBC13G1.09 |
| Gene ID |
21/E10 |
| Gene synonyms/obsolete |
|
| Gene product |
bystin-family protein;
U3 and U14 snoRNA associated protein; involved in rRNA
processing; involved in 40S ribosomal subunit synthesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1393 |
| ORF length (spliced) |
1350 |
| Entry clone length |
1393 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC13G1.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCCAAGGCTCCAAAAAC |
| Rev primer name |
SPBC13G1.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATATTCCATTTCCACAGGA |
| Amino acid length |
449 |
| Molecular weight |
51.3 |
| Isoelectric point (calc.) |
5.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss |