| Gene name |
SPBC1711.08 |
| Gene ID |
21/D12 |
| Gene synonyms/obsolete |
|
| Gene product |
stress-regulated
co-chaperone; activator of Hsp90p; interacts physically with
Swo1p; conserved eukaryotic protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1386 |
| ORF length (spliced) |
1011 |
| Entry clone length |
1386 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
481T:C / 747T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1711.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGGCCACATCTATAAA |
| Rev primer name |
SPBC1711.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACAGAACAGCGCCAAAACCA |
| Amino acid length |
336 |
| Molecular weight |
37.4 |
| Isoelectric point (calc.) |
5.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
moving dots |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |