| Gene name |
SPACUNK4.13c |
| Gene ID |
21/D04 |
| Gene synonyms/obsolete |
|
| Gene product |
GTP-binding protein;
GTP1/OBG family |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1380 |
| ORF length (spliced) |
1224 |
| Entry clone length |
1380 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
546T:C / 950T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPACUNK4.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGTGGTATAGTTACAAG |
| Rev primer name |
SPACUNK4.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACGAGCAATTTTCCAGAAA |
| Amino acid length |
407 |
| Molecular weight |
45.3 |
| Isoelectric point (calc.) |
8.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQIIQEELLL |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
DeltaVision |