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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC3E7.12c
Gene ID 21/C04
Gene synonyms/obsolete
Gene product Chs Four Homologue (pers. comm. Henar Montero); chitin synthase regulatory factor; SEL1 repeat protein; similar to Sp SPBC1289.01C and SPAC24B11.10c and SPCC417.05c
Entry clone Cloned
ORF length (unspliced) 1371
ORF length (spliced)
Entry clone length 1371
No. of intron 0
Sequence status Finished
Sequence results 1265A:C / 1276T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC3E7.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTGCATCCACGTCCAT
Rev primer name SPBC3E7.12.Rv
Rev primer SEQ AGAAAGCTGGGTACTGGAGGAGAACCAAGGTA
Amino acid length 456
Molecular weight 49.5
Isoelectric point (calc.) 6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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