| Gene name |
SPCC162.11c |
| Gene ID |
21/B07 |
| Gene synonyms/obsolete |
|
| Gene product |
uridine kinase; uracil
phosphoribosyltransferase; involved in pyrimidine metabolism;
pyrimidine salvage pathway |
| Entry clone |
Cloned
(Re-cloned) |
| ORF length (unspliced) |
1365 |
| ORF length (spliced) |
|
| Entry clone length |
1365 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC162.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTGAAGCTTTTGAGCG |
| Rev primer name |
SPCC162.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGCATCCATAATACACTTTT |
| Amino acid length |
454 |
| Molecular weight |
51.3 |
| Isoelectric point (calc.) |
7.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPHVVILSL/LLFVKLLEL/LPLNLVQLKI |
| Localization (YFP) |
SPB;
nucleus>cytosol; periphery at site of septum
formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |