| Gene name |
SPAC27F1.07 |
| Gene ID |
21/A01 |
| Gene synonyms/obsolete |
|
| Gene product |
dolichyl-diphospho-oligosaccharide-protein
glycosyltransferase activity; oligosaccharyl transferase
complex; involved in N-linked glycosylation; GPI anchored
protein; glycoprotein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1353 |
| ORF length (spliced) |
|
| Entry clone length |
1353 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
9T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC27F1.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTAGTTCTCAAGCTATT |
| Rev primer name |
SPAC27F1.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGGTGCCCAAATCATGTAG |
| Amino acid length |
450 |
| Molecular weight |
51.6 |
| Isoelectric point (calc.) |
4.8 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLWSIISGLSL/LVYLTTLYL/LRIAIITTLLI |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |