Gene name |
SPBC405.02c |
Gene ID |
20/G08 |
Gene synonyms/obsolete |
SPBC4C3.01 |
Gene product |
hypothetical protein;
serine/proline rich protein; sequence orphan |
Entry clone |
Cloned |
ORF length (unspliced) |
1344 |
ORF length (spliced) |
|
Entry clone length |
1344 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC405.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTTTTGAAAATCTCGG |
Rev primer name |
SPBC405.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACCAGAAACGCCGAGACCA |
Amino acid length |
447 |
Molecular weight |
49.5 |
Isoelectric point (calc.) |
10.2 |
Signal SEQ |
|
No. of transmembrane domain |
2 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LWILASFLPI/LGVAITFLFI |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
DeltaVision |