| Gene name |
SPAC823.11 |
| Gene ID |
20/G06 |
| Gene synonyms/obsolete |
|
| Gene product |
sphingoid
base-phosphate phosphatase; dihydrosphingosine-1-P; putative
sphingolipid metabolism |
| Entry clone |
Cloned in 2006
trial |
| ORF length (unspliced) |
1344 |
| ORF length (spliced) |
1236 |
| Entry clone length |
1344 |
| No. of intron |
2 |
| Sequence status |
Partially
sequenced |
| Sequence results |
100% match in both
ends |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC823.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTCCATAAAAAAAAAAA |
| Rev primer name |
SPAC823.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATATCTTCCACTTTAAGAAG |
| Amino acid length |
411 |
| Molecular weight |
46.8 |
| Isoelectric point (calc.) |
9 |
| Signal SEQ |
|
| No. of transmembrane domain |
7 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRFQLRELIL/LGTHVFFMLAL/LLSLVLLYI/LPPIFKSLRL |
| Localization (YFP) |
no expression clone
|
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
|