Gene name |
SPBC83.14c |
Gene ID |
20/E03 |
Gene synonyms/obsolete |
rfc5 |
Gene product |
replication factor C
(activator 1 subunit); AAA family ATPase; activator of DNA
polymerases |
Entry clone |
Cloned |
ORF length (unspliced) |
1280 |
ORF length (spliced) |
1077 |
Entry clone length |
1280 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
1215T:C /
1268A:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC83.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTTGGTTAGATCAATA |
Rev primer name |
SPBC83.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATAAGAAGGTAACTCCATA |
Amino acid length |
358 |
Molecular weight |
40.4 |
Isoelectric point (calc.) |
9.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol
|
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |