Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC9G1.04
Gene ID 20/C03
Gene synonyms/obsolete oxa101; oxa1; oxa1-1; cox18; oxa1sp1
Gene product respiratory complex biogenesis protein; cytochrome c oxidase assembly protein; localization mitochondrial inner membrane; similar to Sp oxa102 (paralog); essential with oxa102
Entry clone Cloned
ORF length (unspliced) 1266
ORF length (spliced) 1125
Entry clone length 1266
No. of intron 2
Sequence status Finished
Sequence results 317T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC9G1.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTACATTTTCTAATTAG
Rev primer name SPAC9G1.04.Rv
Rev primer SEQ AGAAAGCTGGGTAGTTACTTTGCTTCTTTGAA
Amino acid length 374
Molecular weight 42
Isoelectric point (calc.) 10.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LTTLGVRLAL/LAWFKDLSI
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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