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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1795.03
Gene ID 19/G06
Gene synonyms/obsolete gms1
Gene product UDP-galactose transporter; essential; involved in protein galactosylation (required); essential for the transport of UDP-galactose into the lumen of Golgi apparatus; belongs to the nucleotide-sugar transporter family. SLC35A subfamily; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1237
ORF length (spliced) 1062
Entry clone length 1237
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1795.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTGTCAAGGGCGACGA
Rev primer name SPCC1795.03.Rv
Rev primer SEQ AGAAAGCTGGGTAATGCTTATGATCAACGTCC
Amino acid length 353
Molecular weight 39.1
Isoelectric point (calc.) 9.5
Signal SEQ
No. of transmembrane domain 8
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LTTAIFSILLL/LTFLIGVMLVI
Localization (YFP) nucleus; nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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